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. Author manuscript; available in PMC: 2010 Jan 27.
Published in final edited form as: J Am Chem Soc. 2006 Dec 20;128(50):16131–16137. doi: 10.1021/ja064289h

Figure 3.

Figure 3

Lead(II) probing of radio-labeled Molecule 24 (10 nM) probed in the monomer state and bound to a molar excess of molecule 25 (300 nM). Lanes 1 and 2 are hydroxide and RNase T1 treatments. Lead(II)-induced cleavage (Pb++) was performed as described in Materials and Methods. In the right panel, the secondary structures of molecule 24 and 25 are shown to indicate cognate motifs that interact on dimerization. The cleavage sites in 24 in the monomer and dimer states are indicated with open and solid arrows. Arrows indicate different cleavages within molecule 24.