Table 4.
Confirmation of the putative map positions
| Population | Locus | Chrom. Nr. | Forward and reverse primera | Enzyme used | Confirmation with CAPS markersb |
|---|---|---|---|---|---|
| ver03-392 | CD67 | 6 | F: CCCCTGCAAATCCGTACATA | HpyCH4IV, SsiI | 5/5 R |
| R: CCATACGAGTTGAGGGATCG | 3/3 S | ||||
| ver03-394 | CD67 | 6 | As Ver03-392 | As Ver03-392 | 6/7 R |
| 5/5 S | |||||
| snk7458 | Th21 | 4 | F: ATTCAAAATTCTAGTTCCGCC | MboI | 27/39 R |
| 5/5 S | |||||
| R: AACGGCAAAAAAGCACCAC | |||||
| cap7358 | CP58 | 11 | F: ATGTATGGTTCGGGATCTGG | MspI | 31/32 Rc |
| R: TTAGCACCAACAGCTCCTCT | 18/18 S |
Confirmation with CAPS markers indicates how many of the phenotypically resistant or susceptible individuals were confirmed by the marker analysis
a F: forward primer, R: reverse primer
b R resistant individuals (confirmed/tested), S susceptible individuals (confirmed/tested)
cFor 2 from the 34 resistant individuals the PCR failed so no data on this marker could be retrieved