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. Author manuscript; available in PMC: 2010 Jun 11.
Published in final edited form as: Cell. 2009 Dec 11;139(6):1069–1083. doi: 10.1016/j.cell.2009.11.030

Figure 1. Liganded-AR and Genotoxic Stress Synergistically Induce Chromosomal Translocations in Prostate Cancer Cells.

Figure 1

(A and B) Identification and characterization of induced TMPRSS2:ERGb and TMPRSS2:ETV1b translocations in LNCaP cells. Top: schematic structures for the TMPRSS2, ERG, and ETV1 mRNA indicating exon positions. Middle: RT-PCR amplification of TMPRSS2:ERGb (A) or TMPRSS2:ETV1b (B) fusion transcripts from 48 individual cell samples. Bottom: confirmation of position and fusion sites by automated DNA sequencing. (C and D) Statistical analysis of DHT and IR induced TMPRSS2:ERG (C) and TMPRSS2:ETV1 (D) translocations (n=3, ±SEM). (E and F) Identification and characterization of induced TMPRSS2:ERG (E) and TMPRSS2:ETV1 (F) translocation isoforms in LNCaP cells. Bottom: summary of distinct fusion types. (G and H) Involvement of DNA repair machinery in induced TMPRSS2:ERGb and TMPRSS2:ETV1b translocations by QPCR with indicated siRNAs (n=3, ±SEM).