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. Author manuscript; available in PMC: 2010 Jun 11.
Published in final edited form as: Cell. 2009 Dec 11;139(6):1069–1083. doi: 10.1016/j.cell.2009.11.030

Figure 4. AR-Dependent Local Chromatin Structural Alteration Sensitizes to Site-Specific Genotoxic Stress-Induced DSBs.

Figure 4

(A-E) Top: schematic diagram showing the relative positions of break/fusion sites and potential AREs located on ERG and TMPRSS2 loci. Blue boxes, potential AREs; red boxes, break/fusion sites; black arrows, relative positions of PCR primers. Bottom: LNCaP cells were treated with DHT (10−7 M) (A), DHT (10−7 M) (B), IR (50 Gy) (C) or both (D) and DHT (10−7 M) (E) for time courses as indicated. ChIP analyses were performed with indicated antibodies on indicated regions (n=2, ±SEM). (F) Examination of TMPRSS2:ERGb and TMPRSS2:ETV1b fusion transcripts with RPA2 siRNAs (n=3, ±SEM).