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. Author manuscript; available in PMC: 2010 Jan 27.
Published in final edited form as: Clin Immunol. 2008 Oct 8;129(3):482–491. doi: 10.1016/j.clim.2008.08.022

FIGURE 2.

FIGURE 2

FIGURE 2

A. Phenotype of unpulsed-DC (UP-DC), KLH pulsed-DC (KLH-DC), cryopreserved KLH pulsed-DC (C-KLH-DC) and LPS pulsed-DC (LPS-DC). UP-DC, KLH-DC, C-KLH-DC and LPS-DC were stained using fluorochrome-conjugated monoclonal antibodies (mAbs) for MHC class I, MHC class II, CD80, CD86, CD40 and CD11c and analyzed by flow cytometry. Thin lines represent staining with matched isotype control mAbs, thick lines represent staining with the designated mAbs. Numbers in histogram plots designate percentage of positive cells and geometrical mean of fluorescence intensity of positive cells, respectively. B. Cytokine secretion by UP-DC, KLH-DC, C-KLH-DC and LPS-DC. Supernatants of DC cultures were analyzed for IL-12p70 and IL-10 content using ELISA. These experiments were repeated three times with similar results.