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. Author manuscript; available in PMC: 2010 Jan 27.

Published in final edited form as: Clin Immunol. 2008 Oct 8;129(3):482–491. doi: 10.1016/j.clim.2008.08.022

A. Allogeneic splenocyte stimulatory capacity of unpulsed-DC (UP-DC), KLH pulsed-DC (KLH-DC) and cryopreserved KLH pulsed-DC (C-KLH-DC). Allogeneic splenocytes (2 × 10⁵ per well), as responders, were incubated with or without increasing numbers of each type of DC as stimulators. Proliferation was measured after 5 days of co-culture using a WST-1-based assay as described in the Materials and Methods. Data are reported as the average absorbance ± SD of triplicate samples. * P < 0.001 versus UP-DC and KLH-DC. B. Cytokine release capacity of UP-DC, KLH-DC and C-KLH-DC upon induction of maturation. Supernatants of DC cultured in the presence of LPS were analyzed for IL-12p70 and IL-10 content using ELISA. * P < 0.05 versus UP-DC and KLH-DC. These experiments were repeated at least two times with similar results.

A. Allogeneic splenocyte stimulatory capacity of unpulsed-DC (UP-DC), KLH pulsed-DC (KLH-DC) and cryopreserved KLH pulsed-DC (C-KLH-DC). Allogeneic splenocytes (2 × 10⁵ per well), as responders, were incubated with or without increasing numbers of each type of DC as stimulators. Proliferation was measured after 5 days of co-culture using a WST-1-based assay as described in the Materials and Methods. Data are reported as the average absorbance ± SD of triplicate samples. * P < 0.001 versus UP-DC and KLH-DC. B. Cytokine release capacity of UP-DC, KLH-DC and C-KLH-DC upon induction of maturation. Supernatants of DC cultured in the presence of LPS were analyzed for IL-12p70 and IL-10 content using ELISA. * P < 0.05 versus UP-DC and KLH-DC. These experiments were repeated at least two times with similar results.