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. 2009 Nov 20;192(3):643–656. doi: 10.1128/JB.00700-09

FIG. 6.

FIG. 6.

Gene organization of the loci identified by mini-Tn5 mutagenesis of Alcanivorax strains. The diagram shows the localization of mini-Tn5 insertions in the mutants of A. jadensis T9 (Aj17) and of A. borkumensis SK2 (ABO) showing a defect in lipid export. The positions of mini-Tn5 insertions in the respective mutants (see Table 3 for designations) are indicated by triangles. Lengths and directions of arrows show the genes and directions of transcription of the respective genes. For Aj17, putative identities of the gene products suggested by amino acid sequence identities to protein in the GenBank database are as follows: blaB, metallo-beta-lactamase family protein; MgCo, CorA family magnesium/cobalt transporter; DNAr, possibly reparation of alkylated DNA. For A. borkumensis, the gene products are tnpB, transposon B protein; czcA2, heavy metal RND efflux transporter, CzcA family; czcB2, heavy metal RND efflux membrane fusion protein, CzcB family; and czcC2, heavy metal RND efflux outer membrane protein, CzcC family (mutant ABO_6/39). alg8, alginate biosynthesis protein alg8; alg44, alginate biosynthesis protein Alg44; algK, alginate biosynthesis regulator; algE, outer membrane alginate export protein; algI, alginate O-acetylation protein AlgI; algJ, alginate O-acetylation protein AlgJ; algF, alginate O-acetyltransferase; algX, alginate biosynthesis protein AlgX; algL, alginate lyase; algG, poly(beta-d-mannuronate) C5 epimerase precursor; algA, mannose 6-phosphate isomerase/mannose 1 phosphatase (mutants ABO_10/30; ABO_19/48 and ABO_26/1). znuA, high affinity zinc uptake system protein znuA precursor; znuC, zinc transport protein, ATPase; znuB, zinc ABC transporter permease protein; MATE, MATE efflux family protein putative (mutant ABO_25/21). amtB2, ammonium transporter, putative; hyp, hypothetical protein; gltP, sodium dicarboxylate symporter family protein; oscd, oxidoreductase, short-chain dehydrogenase/reductase family (mutant ABO_27/29). Nas, Na+ symporter family protein; hyp, hypothetical protein; tsp, two-component sensor protein; tr, transcription regulator, putative; rr, response regulator; shk, sensor histidine kinase; RND, probable RND efflux transporter (mutant ABO_27/56).