TABLE 2.
Oligonucleotides used in this work as primers for PCR and to sequence recombinant plasmids
| Primer | Sequencea |
|---|---|
| I_end | GGCCGCACTTGTGTA TAAGAGTCAG |
| O_end | GCGGCCAGATCTGATCAAGAGACAG |
| M13-forward | GTAAAACGACGACGGCCGT |
| M13-reverse | CAGGAAACAGCTATGAC |
| 5′_algL | AAGGATCCGTGTTGGTTACCGGGTGTTCGCAAGC |
| 3′_algL | AAAAAGCTTTTACGGAGTCGCTTGCCACAATCCCG |
| 5′_algE | AAAAAGCTTGTGTCGCGATGTCTTTCTAATTCCGCCAG |
| 3′_algE | AAAAAGCTTTCACCACCTCCCTGCGACAACAAACAG |
| 5′_MATE | TTTTGGATCCAAGGAGAATATATGAGTGTGACCCTC |
| 3′_MATE | TTTTTAAGCTTTCATGCATGATGGCGACGTC |
| 5′_SmR | AAAAAGTCGACCTCACGCCCGGAGCGTAGCGACC |
| 3′_SmR | AAAAAGTCGACAACGACCCTGCCCTGAACCGACG |
a
Restriction sites used for cloning purposes are underlined.