FIG. 7.

Partial digestion of Gli521. (A) Protein profiles of Gli521 digested with various concentrations of proteinase K. Gli521 (0.3 μg) was treated with 0.015, 0.06, 0.15, 0.3, and 0.6 μg proteinase K and subjected to SDS-PAGE. The resultant peptide fragments, fragments a to d, were identified by peptide mass fingerprinting. (B) Mapping of digests on the amino acid sequence. The N-terminal 43 amino acids predicted from the genetic information are processed in the mature form. The N-terminal amino acid in each fragment was determined by Edman analysis. The C-terminal positions were based on the C-terminal end of the peptide detected by peptide mass fingerprinting. (C) Schematic diagram of Gli521 domains. Gli521 consists of three domains, domains I, II, and III. The molecular mass (in kDa) is indicated below each domain. TM, transmembrane segment.