FIG. 5.

A single amino acid substitution in FBPase confers increased resistance to propionate. Growth curves were determined in NCE minimal medium; carbon and energy sources were succinate (30 mM) and propionate ranging from 30 mM to 1 mM. Strain JE11237 (prpC iolR fbp121 [Fbp(S123F)]) grew in the presence of as much as 30 mM propionate. The control isogenic strain JE10831 (prpC iolR fbp⁺) was grown under the same conditions as strain JE11237. A no-propionate, succinate-only control is shown to indicate growth rates for the strains when propionate was absent. No significant difference was observed when the strains were grown on succinate.