TABLE 1.
Strain or plasmid | Genotypea | Source or reference |
---|---|---|
Bacterial strains | ||
E. coli | ||
BL21(λDE3) | F−ompT hsdSB(rB− mB+) dcm gal (DE3) | New England Biolabs |
DH5α/F′ | F′/endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacZYA-argF)U169 deoR [φ80dlacD(lacZ)M15] | New England Biolabs |
S. enterica | ||
TR6583 | metE205 ara-9 | K. Sanderson via J. Roth |
Derivatives of TR6583 | ||
JE2170 | prpC114::MudJ | 23 |
JE4175 | pBAD30 | Lab collection |
JE4199 | prpC114::MudJ/pPRP35 | 54 |
JE4440 | prpC114::MudJ/pBAD30 | 54 |
JE8431 | ΔprpC134::cat+ | |
JE8571 | ΔprpC254 | |
JE10609 | prpC114::MudJ/pFBP2 | |
JE10713 | iolR1::cat+ | Lab collection |
JE10831 | prpC114::MudJ ΔiolR1::cat+ | |
JE11237 | prpC114::MudJ ΔiolR1::cat+fbp121 | |
JE12230 | ΔprpC254 gltA1182::MudJ | |
JE12239 | ΔprpC254/pBAD30 | |
JE12240 | ΔprpC254/pGLTA2 | |
JE12241 | ΔprpC254 gltA1182::MudJ/pBAD30 | |
JE12242 | ΔprpC254 gltA1182::MudJ/pGLTA2 | |
JE12243 | ΔprpC254 gltA1182::MudJ/pPRP35 | |
JE12246 | ΔprpC254/pPRP35 | |
JE12516 | prpC114::MudJ/pAMN1 | |
JE12597 | pAMN1 | |
JE11377 | prpD126::cat+gltA1182::MudJ | |
JE11392 | prpD126::cat+gltA1182::MudJ/pPRP35 | |
JE11393 | prpD126::cat+gltA1182::MudJ/pGLTA2 | |
JE11394 | prpD126::cat+gltA1182::MudJ/pBAD30 | |
JE11397 | prpC114::MudJ/pGLTA2 | |
Plasmids | ||
pBAD30 | Cloning vector; pACYC184 origin of replication, bla+ (Ampr); expression of the gene of interest under the control of the arabinose-inducible ParaBAD promoter | 22 |
pFBP2 | fbp+ cloned into pBAD30 | |
pGLTA2 | gltA+ cloned into pBAD30 | 28 |
pPRP35 | prpC+ cloned into pBAD30 | 54 |
pAMN1 | amn+ cloned into pBAD30 |
MudJ is an abbreviation of MudI1734 (13).