TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| BL21(λDE3) | F−ompT hsdSB(rB− mB+) dcm gal (DE3) | New England Biolabs |
| DH5α/F′ | F′/endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacZYA-argF)U169 deoR [φ80dlacD(lacZ)M15] | New England Biolabs |
| S. enterica | ||
| TR6583 | metE205 ara-9 | K. Sanderson via J. Roth |
| Derivatives of TR6583 | ||
| JE2170 | prpC114::MudJ | 23 |
| JE4175 | pBAD30 | Lab collection |
| JE4199 | prpC114::MudJ/pPRP35 | 54 |
| JE4440 | prpC114::MudJ/pBAD30 | 54 |
| JE8431 | ΔprpC134::cat+ | |
| JE8571 | ΔprpC254 | |
| JE10609 | prpC114::MudJ/pFBP2 | |
| JE10713 | iolR1::cat+ | Lab collection |
| JE10831 | prpC114::MudJ ΔiolR1::cat+ | |
| JE11237 | prpC114::MudJ ΔiolR1::cat+fbp121 | |
| JE12230 | ΔprpC254 gltA1182::MudJ | |
| JE12239 | ΔprpC254/pBAD30 | |
| JE12240 | ΔprpC254/pGLTA2 | |
| JE12241 | ΔprpC254 gltA1182::MudJ/pBAD30 | |
| JE12242 | ΔprpC254 gltA1182::MudJ/pGLTA2 | |
| JE12243 | ΔprpC254 gltA1182::MudJ/pPRP35 | |
| JE12246 | ΔprpC254/pPRP35 | |
| JE12516 | prpC114::MudJ/pAMN1 | |
| JE12597 | pAMN1 | |
| JE11377 | prpD126::cat+gltA1182::MudJ | |
| JE11392 | prpD126::cat+gltA1182::MudJ/pPRP35 | |
| JE11393 | prpD126::cat+gltA1182::MudJ/pGLTA2 | |
| JE11394 | prpD126::cat+gltA1182::MudJ/pBAD30 | |
| JE11397 | prpC114::MudJ/pGLTA2 | |
| Plasmids | ||
| pBAD30 | Cloning vector; pACYC184 origin of replication, bla+ (Ampr); expression of the gene of interest under the control of the arabinose-inducible ParaBAD promoter | 22 |
| pFBP2 | fbp+ cloned into pBAD30 | |
| pGLTA2 | gltA+ cloned into pBAD30 | 28 |
| pPRP35 | prpC+ cloned into pBAD30 | 54 |
| pAMN1 | amn+ cloned into pBAD30 |
a
MudJ is an abbreviation of MudI1734 (13).