FIG. 1.

The schematic representation of Tn1549 and of its functional modules. Coordinates of the 3,150-bp fragment including the mobilization module are indicated according to GenBank accession no. AF192329.1. Open arrows represent ORFs and indicate the sense of transcription. Large stem-loops are indicated. The relaxase gene cluster is enlarged and inverted to facilitate analysis. The primers used for amplification of transgene fragments from the cDNA are indicated by arrowheads. The resulting PCR products are shown by a thin dark line, which corresponds to a cotranscription. The inserts of plasmids indicated on the right are represented by horizontal bars. Initiation codons of orf29 and orf30 are italicized. The beginning of transcription of orf30 and of the mobilization operon detected by 5′ RACE are indicated in bold, and the −35 and −10 motifs are boxed.