FIG. 2.

Mapping of the 5′ end of the nmb1869-specific transcript (A) and the fhbp-specific transcript (B) by primer extension. Total RNA (20 μg) prepared from cultures of the wild-type strain (MC58) grown to mid-logarithmic phase was hybridized with gene-specific primers (741PE1 and 1869PE1) (Table 1) and elongated with reverse transcriptase. Sequence reactions (G, A, T, and C) were performed with the same primers on the respective cloned promoter regions and run in parallel. The elongated primer bands mapping the major 5′ end of the corresponding gene transcripts are indicated. The corresponding +1 nt of transcriptional initiation and the upstream −10 and −35 promoter elements are underlined and in bold face in the nucleotide sequences of the respective intergenic regions shown underneath. An FNR box located at −40.5 bp is highlighted in gray. The pentanucleotide ATATT (in the box) is the target of insertion for a 181-bp AT-rich nucleotide sequence (ATR) in the genome of MenA strain Z2491 (37).