TABLE 1.
Oligonucleotides used in this study
| Primer | Sequencea | Site |
|---|---|---|
| FNR-for1 | attagcatatgGCTTCGCATAATACTACAC | NdeI |
| FNR-rev2 | attagatgcatCAAATGGCGTGCGAGC | NsiI |
| D148A-F | GTGAAATCGTGCGCGCCCAAGGTGTTATGCTG | |
| D148A-R | CAGCATAACACCTTGGGCGCGCACGATTTCAC | |
| 1869-1 | attaggaattcGTACATCAGTTCGAACAGGGCGTGG | EcoRI |
| 1869-2 | TGACGATTTGcccgggCTTGGGCAATAGGTAAATAAGGCGG | SmaI |
| 1869-3 | CTATTGCCCAAGcccgggCAAATCGTCAAATAACAGGTTGC | SmaI |
| 1869-4 | attcagaagcttCTGAAAGGCGGTTAAGGCGGAATGG | HindIII |
| 741FOR | CAAATCGAAGTGGACGGGCA | |
| 741REV | TGTTCGATTTTGCCGTTTCCCTG | |
| 1869rtF | CAACTGGGCTTCTCCTCTGT | |
| 1869rtR | ATTTCGCCTTCAACGGATAC | |
| 741Pf | CGTCAAATAACAGGTTG | |
| 741PE1 | GAATCAGGGCAGTGGTCAGAG | |
| 1869FW | CCAACTGCTTGATCATGCTGC | |
| 1869rev | GTGTGGAAATTCTTCGACAGCC | |
| 741F2 | TTGGCTGCGAAGGTCAGGCAG | |
| 741REV2 | GTATGTTCGCCCGCTATGTCG | |
| IG1871F | CATCAGCGGTTCCGTCCTTTAC | |
| IG1871R | GCGGATTTCCGGCAGAATCAG | |
| FNR-rev1 | attagggatccTCAAATGGCGTGCGAGC | BamHI |
| 1869PE1 | GCAGCATGATCAAGCAGTTG | |
| 1869 PF | TCATGGAGGCTGCAGACCAAG | |
| 1869 PR-2 | GCGAGCCGTCCATCATTACAG |
a
Capital letters indicate N. meningitidis-derived sequences, lowercase letters indicate sequences added for cloning purposes, and underlined letters indicate restriction enzyme recognition sites.