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Complementation of ST73. H37Rv (⧫), ST73 (•), and ST73 transformed with a plasmid expressing irtA (▴), irtB (▪), or irtAB (×) were inoculated in LIMM. Growth was monitored each day by measuring the OD540. The results of a representative experiment are shown. The experiment was repeated three times. All strains grew normally in high-iron medium (data not shown).
