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Spectral analysis of IrtA-NTD. (A) UV/visible spectrum of purified IrtA-NTD. An inset shows an expanded-scale spectrum of characteristic flavin absorbance between 300 and 500 nm. (B) UV/visible spectrum of the filtrate recovered after Gnd-HCl denaturation of IrtA-NTD and filtration through a Microcon YM-10 column.
