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Distribution of DamX protein tagged with a 3× FLAG epitope in subcellular fractions of S. enterica serovar Typhimurium. Anti-FLAG western hybridization is shown for three fractions: the outer membrane (OM), the inner membrane (IM), and the cytosol (Cyt). The volumes loaded for all fractions were normalized to the same number of bacteria (5 × 107 CFU). Prestaining molecular mass standards in kDa are indicated.
