TABLE 1.
MICs of sodium deoxycholate in S. enterica strains carrying dam and damX deletion alleles
| Strain | Genotype | MIC of DOC (%)a |
|---|---|---|
| ATCC 14028 | wt | 6.0 |
| SV4536 | Δdam-230 | 0.2 |
| SV5116 | ΔdamX1 | 0.4 |
| SV5307 | ΔdamX2 | 0.4 |
| SV5441 | DUP [ΔdamX2/ΔdamX2] | 0.2 |
| SV5443 | DUP [ΔdamX2/dam+] | 4.0 |
| SV5538 | ΔdamX2 ΔasmA::Kmr | 6.0 |
| SV5539 | ΔdamX2 ΔmutH::Kmr | 0.4 |
a
MICs are medians of results from >5 independent determinations. Overnight cultures in LB were diluted 1:50 in fresh LB and incubated at 37°C with shaking until late exponential phase (OD660 = 0.6 to 0.8). Cultures were then diluted to obtain a concentration of 3,000 cells/ml. Aliquots were transferred to microtiter plates whose wells had previously been filled with aqueous solutions of DOC (0.2%, 0.4%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, and 7%). After overnight incubation at 37°C, bacterial growth was visually monitored.