Figure 1.

Expression profile of Wnt ligands and Wnt-related genes in human trabecular meshwork cells. Total RNA extracted from cells was reverse transcribed and amplified with specific primers for Wnt-related genes listed in Table 1. PCR products were resolved on a 1% agarose gel and visualized by ethidium bromide staining. Positive PCR products were obtained for Wnt2b, Wnt3, Wnt5a, Wnt5b, Dsh1, Dsh2, Dsh3, Fzd1, Fzd2, Fzd4, Fzd5, Fzd7, LRP5, LRP6, CTNNB1, GSK3β, APC, TCF3, TCF4, TCF7, Dkk1, Dkk2, sFRP1, sFRP2, and sFRP3. Negative controls with RNA samples that were not subjected to reverse transcription were included for all PCR reactions. No PCR products were seen with any of the negative controls (representative results are shown for Wnt2b and Wnt5a). When applicable, positive controls, using MCF-7 (shown for Wnt3 and Wnt5b) and HEK293 cDNAs, were performed in parallel. All PCR products were confirmed by sequence analyses, and all the experiments were performed in at least two different cell lines from two different donors.