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. 2010 Jan 28;16:122–129.

Table 1. Primer pair sequences, annealing temperature, and product sizes of Wnt-related genes.

Genes examined Forward primer Reverse primer Annealing temperature (°C) Expected product size (bp)
Wnt1
5’-TCCTCCACGAACCTGCTTAC-3’
5’-GCCTCGTTGTTGTGAAGGTT-3’
54
491
Wnt2
5’-GGTGATGTGCGATAATGTGC-3’
5’-GCCAGCTCTGTTGTTGTGAA-3’
52
404
Wnt2b
5’-AAGATGGTGCCAACTTCACC-3’
5’-GGCCACAGCACATGATTTCAC-3’
54
188
Wnt3
5’-CTGTGAGGTGAAGACCTGCTG-3’
5’-GATGCAGTGGCATTTTTCCT-3’
54
357
Wnt3a
5’-GGTGGCTGTAGCGAGGACAT-3’
5’-ATGCCGTGCGAGCTGACGTT-3’
56
454
Wnt4
5’-GCATCTCAGAGGAGGAGACG-3’
5’-TCAGAGCATCCTGACCACTG-3’
56
363
Wnt5a
5’-ATTTTTCTCCTTCGCCCAGGT-3’
5’-GGCTCATGGCGTTCACCAC-3’
55
358
Wnt5b
5’-CCAAAGGATCAGAGGAGCAG-3’
5’-TACACCTGACGAAGCAGCAC-3’
56
483
Wnt6
5’-TGGTTATGGACCCTACCAGCA-3’
5’-CGTCCATAAAGAGCCTCGAC-3’
54
467
Wnt7a
5’-CTGGAGGAGAACATGAAGC-3’
5’-ACAGCACATGAGGTCACAGC-3’
54
354
Wnt8a
5’-TCCCAAGGCCTATCTGACCTAC-3’
5’-CCGGCCCTGTTGTTGTGA-3’
57
407
Wnt10b
5’-AATGCGAATCCACAACAACA-3’
5’-ACAGCACATAGCAGCACCAG-3’
54
448
Wnt11
5’-CTACACAACAGTGAAGTG-3’
5’-CCCACCTTCTCATTCTTCATGC-3’
55
298
Dsh1
5’-CACCCTGAACCTCAACAGTGG-3’
5’-CCCTTCACTCTGCTGACTCC-3’
56
201
Dsh2
5’-CCTTCAGCAGCGTCACAGATTCC-3’
5’-AGTGGGCAGCAGGGGCC-3’
59
895
Dsh3
5’-CAGCCCCCTTCTGTGCTGATAA-3’
5’-AGAAGGTGATCTTGTTGA-3’
59
1196
Fzd1
5’-TGCGAGGCGCTCATGAACAA-3’
5’-CCTCGGCGAACTTGTCATTA-3’
54
570
Fzd2
5’-CGTCCTCAAGGTGCCATCCTA-3’
5’-CAGCCCGACAGAAAAATGAT-3’
54
248
Fzd4
5’-CTCGGCTACAACGTGACCAAGAT-3’
5’-AATATGATGGGGCGCTCAGGGTA-3’
57
604
Fzd5
5’-CTGCTACCAGCCGTCCTTCAGT-3’
5’-CCATGCCGAAGAAGTAGACCA-3’
59
319
Fzd7
5’-CGACGCTCTTTACCGTTCTC-3’
5’-GCCATGCCGAAGAAGTAGAG-3'
54
246
Fzd8
5’-GGACTACAACCGCACCGACCT-3’
5’-ACCACAGGCCGATCCAGAAGAC-3’
59
406
LRP5
5’-AAGATCATTGTGGACTCGGAC-3’
5’-GAAAGGCTCGCTTGGG-3’
52
395
LRP6
5’-ACTGTATCCCTGTGGCTTGG-3’
5’-CCCTTCATACGTGGACACA-3’
54
426
CTNNB1
5’-CATGGAACCAGACAGAAAAGC-3’
5’-GCTACTTGTTCTTGAGTGAAG-3’
52
200
GSK3β
5’-GCAGCAGCCTTCAGCTTTTGG-3’
5’-CCGGAACATAGTCCAGCACCAG-3’
59
358
APC
5’-TCCACAACATCATTCACTCACAG-3’
5’-TGCTCGCCAAGACAAATTCC-3’
53
505
TCF1
5’-GAGCAAAGAGGCACTGATCC-3’
5’-CTGGTTGAGGCCAGTGGTAT-3’
54
361
TCF3
5’-GGGTCTTCCATCCTCGGTGTA-3’
5’-GAGTAGATCGAGGCCAGTGC-3’
56
488
TCF4
5’-GTTTGTATTTTTTGGCG-3’
5’-GAATGGCTGCCTTAGGG-3’
49
467
TCF7
5’-GAGCCAAGGTCATTGCAGAGT-3’
5’-GTGGTGGATTCTTGGTGCTT-3’
54
222
Dkk1
5’-GATCTGTAAACCTGTCCT-3’
5’-GAAGAATTACTGGCTTGATG-3’
50
149
Dkk2
5’-CTGATGGTGGAGAGCTCACAG-3’
5’-ATTATTGCAGCGGGTACTGG-3’
56
311
sFRP1
5’-CAACCTGCTGGAGCACGAGAC-3’
5’-CGCTGGCACAGAGATGTTCA-3’
58
379
sFRP2
5’-CACGGCATCGAATACCAGAAC-3’
5’-GATGCAAAGGTCGTTGTCCT-3’
58
308
sFRP3 5’-GGGCTGTGAGCCCATACTCAT-3’ 5’-GGCAGCCAGAGCTGGTATAG-3’ 57 379