Table 2. Wnt components identified in human trabecular meshwork (TM) cells.

Components in Wnt pathway	Genes examined	Genes identified
Wnt ligand	Wnt1, Wnt2*, Wnt2b, Wnt3, Wnt3a*, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt8a, Wnt10b*, Wnt11*	Wnt2b, Wnt3, Wnt5a, Wnt5b
Transduction protein	Dsh1, Dsh2, Dsh3	Dsh1, Dsh2, Dsh3
Receptor	Fzd1, Fzd2, Fzd4, Fzd5, Fzd7, Fzd8, LRP5, LRP6	Fzd1, Fzd2, Fzd4, Fzd5, Fzd7, LRP5, LRP6
β-Catenin and Degradation complex	CTNNB1, GSK3β, APC	CTNNB1, GSK3β, APC
Transcription factor	TCF1*, TCF3, TCF4, TCF7	TCF3, TCF4, TCF7
Inhibitor	Dkk1, Dkk2, sFRP1, sFRP2, sFRP3	Dkk1, Dkk2, sFRP1, sFRP2, sFRP3

RT–PCR experiments were performed to detect Wnt-related gene transcripts. Primers used for the analyses are given in Table 1. The asterisk indicates that positive PCR products were obtained using MCF-7 or HEK293 cDNAs but not with cDNA from human TM cells.