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. 2010 Jan 28;5(1):e8930. doi: 10.1371/journal.pone.0008930

Figure 1. Segregation and EP bridge architecture in EPs/ECs.

Figure 1

A–B: Trichome staining showed EP islands surrounded by ECs. C: CD31 (green), E-cadherin (red), and nucleus (blue) immunostaining of distinct boundaries between cell types. D: Confocal imaging of an EP island immunostained for E-cadherin (green) and nuclei (blue) with 3D reconstruction shown in an x-z section. E: EP island barriers were composed of collagen IV (green) F: EP bridges with (Fi) or without nuclei (Fii) connected EP islands. G–H: Trichrome staining of EP bridges with nuclei (E) and without nuclei (F). I–K: F-actin (red), nucleus (blue), and acetylated α-tubulin (green) immunostaining for microtubules showed EP bridge structural components. L: x-z section of an EP bridge from confocal 3D reconstruction. Scale bars: (A), 500 µm; (B–E, Fi–Fii), 50 µm; (F), 250 µm; (G–K), 50 µm; (Gi, Hi–Hiii, Ii), 10 µm.