Table 1. Comparison of the M1 genome features with methanogens from the order Methanobacteriales.
M. ruminantium M1 | M. smithii PS [34] | M. smithii ALIa | M. smithii F1a | Methanothermobacter thermoautotrophicus ΔH [91] | Methanosphaera stadtmanae MCB-3 [20] | |
Source | Bovine rumen | Sewage digester | Human | Human faeces | Sewage sludge | Human faeces |
Project status | complete | complete | draft | draft | complete | complete |
Genome size (bp) | 2,937,203 | 1,853,160 | 1,704,865 | 1,707,624 | 1,751,377 | 1,767,403 |
G+C content (%) | 33 | 31 | 31 | 31 | 50 | 28 |
Number of ORFs | 2217 | 1795 | 1709 | 1710 | 1873 | 1534 |
Coding area (%) | 81 | 90 | 90 | 90 | 90 | 84 |
rRNA operons | 2 | 2 | nd | nd | 2 | 4 |
tRNAs (with intron) | 58 (2) | 34 (1) | 34 | 34 | 39 (3) | 40 (1) |
Non-coding RNA | 3 | 3 | nd | nd | 2 | 2 |
Insertion sequences | 4 | 8 | nd | nd | 0 | 4 |
Prophage | Yes | Yes | nd | nd | No | No |
CRISPR regions | 2 | 1 | nd | nd | 2 | 2 |
Adhesin-like proteins | 105 | 48 | nd | nd | 0 | 37 |
LPxTG motif | 1 | 2 | nd | nd | 0 | 0 |
Sortases | 1 | 1 | nd | nd | 2 | 0 |
Draft genome data obtained from National Centre for Biotechnology Information http://www.ncbi.nlm.nih.gov/;
Data not determined (nd) from draft genome.