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. 2010 Feb;135(2):135–147. doi: 10.1085/jgp.200910334

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© 2010 Villalonga et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Voltage-dependent K⁺ currents in Raw 264.7 macrophages. (A) Representative traces of delayed rectifier K⁺ currents in control, LPS-, and DEX-treated cells. Macrophages were treated with 100 ng/ml LPS and 1 µM DEX for 24 h. Cells were held at −80 mV, and currents were elicited by a depolarizing pulse to +60 mV (250-ms duration). Black trace, control; dark gray trace, LPS; light gray trace, DEX. (B) Current density (pA/pF) versus voltage relationship of K⁺ currents. Current density was calculated as a function of peak amplitude and the cell capacitance of each recorded cell. (C) Peak current density at +60 mV. (D) Cell capacitance (pF) from the same cell population from B. (E) Channel density was calculated from the conductance of K_v1.3 (13 pS) and K_v1.5 (8 pS) following the real-time PCR results in Fig. 4 and the K_v1.3/ K_v1.5 ratio shown in Fig. 8. (F) The results in E as a function of the plasma cell membrane surface. White (bar or circle), control; black (bar or circle), LPS; gray (bar or circle), DEX. *, P < 0.05; ***, P < 0.001 versus control; n > 10 cells per group (Student’s t test).