Figure 4.
Activation and immunosuppression differentially regulates Kv1.3 and Kv1.5 in macrophages. Control cells were incubated for 24 h in the presence of LPS and DEX. Samples were collected after the addition of agents, and Kv1.3 and Kv1.5 expression was analyzed. (A) Kv1.3 (white bar) and Kv1.5 (black bar) relative mRNA expression by real-time PCR. Values are the mean ± SEM (n = 4). Significant differences were only found with Kv1.3 (*, P < 0.05; **, P < 0.01 vs. control; Student’s t test). Using standard curves, fold variation in arbitrary units (A.U.) was normalized to 18S relative quantity (RQ) as follows: (Kv1.5 or Kv1.3 RQ (LPS or DEX)/18S RQ (LPS or DEX))/(Kv1.5 or Kv1.3 RQ (control)/18S RQ (control)). (B) Kv1.3, Kv1.5, and iNOS protein expression in macrophages. Representative blots are shown. (C and D) Kv1.3 (C) and Kv1.5 (D) quantification of data in B. Values were normalized to β actin. In all cases, arbitrary units are standardized to the control value in the absence of treatment. White bars, control; black bars, LPS; gray bars, DEX. Values are the mean ± SEM of at least three independent experiments. *, P < 0.05; **, P < 0.01 versus control (Student’s t test).