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. 2010 Jan 25;188(2):223–235. doi: 10.1083/jcb.200910042

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© 2010 Bernasconi et al.

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Figure 4. — Consequences of HRD1 and GP78 down-regulation on degradation of membrane-tethered and soluble variants of BACE476 and CD3-δ. (A) The efficiency of siRNA-based HRD1 and GP78 down-regulations were checked by immunoblotting. Tubulin is a loading control. (B) Radiolabeled BACE476 was immunoisolated after the indicated chase times from cells expressing a scrambled siRNA (siSCR; lanes 1–3), an siRNA targeting HRD1 (siHRD1; lanes 4–6), GP78 (siGP78; lanes 7–9), or both HRD1 and GP78 (siHRD1/siGP78; lanes 10–12). Relevant bands were quantified and plotted. (C–E) Same as described in B for BACE476Δ, CD3-δ, and CD3-δΔ, respectively. Molecular mass markers are shown on the left for all gels (given in kilodaltons).