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. 2010 Jan 25;188(2):223–235. doi: 10.1083/jcb.200910042

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© 2010 Bernasconi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Disposal of soluble misfolded polypeptides relies on SEL1L and both OS-9 + XTP3-B. (A) The efficiency of siRNA-based SEL1L, OS-9, and XTP3-B down-regulations were checked by immunoblotting. Tubulin is a loading control. (B) Radiolabeled BACE476 was immunoisolated after the indicated chase times from cells expressing a scrambled siRNA (siSCR; lanes 1–3), an siRNA targeting SEL1L (siSEL1L; lanes 4–6), OS-9 (siOS-9; lanes 7–9), XTP3-B (siXTP3-B; lanes 10–12), or both XTP3-B and OS-9 (siXTP3-B/siOS-9; lanes 13–15). Relevant bands were quantified and plotted. (C–E) Same as described in B for BACE476Δ, CD3-δ, and CD3-δΔ, respectively. Molecular mass markers are shown on the left for all gels (given in kilodaltons).