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. 2010 Jan 18;207(1):189–206. doi: 10.1084/jem.20091964

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© 2010 Henri et al.

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Figure 2. — Expression of EpCAM, CD24, F4/80, and SIRPα on the five skin DC subsets before and after their migration to CLNs. DC subsets were prepared as specified in Figs. 1 and 3 from dermis and CLNs from B6 (CD45.1)→B6 (CD45.2) BM chimeras and analyzed for the expression of EpCAM, CD24, F4/80, and SIRPα. Isotype control staining is shown by the shaded histograms. In steady-state skin, LCs and CD207⁻ CD11b⁺ DDCs were homogeneously F4/80⁺. CD207⁻ CD11b⁻ DDCs showed a heterogeneous pattern of F4/80 expression, whereas both subsets of CD207⁺ DDCs were F4/80⁻. Upon migration to the CLNs, F4/80 expression was down-regulated on mLCs and CD207⁻ mDDCs. If we except mLCs that showed a slight decrease in Sirpα levels, Sirpα expression was up-regulated or induced on all the remaining skin-derived migratory DC subsets. Data shown are representative of at least six chimeric mice corresponding to three independent experiments.