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. 2010 Jan 18;207(1):189–206. doi: 10.1084/jem.20091964

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© 2010 Henri et al.

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Figure 4. — The five skin DC subsets proliferate before their migration to LNs. (A–C) The five skin DC subsets (A and B) and their migratory counterparts found in the CLNs (C) were identified using B6 (CD45.1)→B6 (CD45.2) chimeras, as specified in Figs. 1 and 3, and analyzed for Ki-67 expression. Lymphoid tissue–resident DCs were defined on the basis of their CD11c^high MHCII^inter phenotype (Fig. S3). Positioning of the Ki-67⁺ gate is based on staining with isotype control antibody (Fig. S4). Data shown are representative of three independent experiments.