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. 2010 Jan 18;207(1):223–235. doi: 10.1084/jem.20091279

Figure 1.

Figure 1.

Growth of B16 mouse melanoma cells in collagen-fibrin gels. (A) Collagen-fibrin gels containing 5 × 104 (▴), 5 × 105 (■), or 5 × 106 (•) B16 cells/ml and RPMI 1640 with 10% FBS and 5 × 10−5 M β-ME were incubated at 37°C for 3 d. Gels were harvested daily and their content of clonogenic B16 cells was assessed as described in Materials and methods. Data shown represent mean ± SEM of n = 3 experiments performed in duplicate. (B) Hematoxylin/eosin-stained frozen sections of B16 cells grown at 37°C in collagen-fibrin gels for 1, 3, and 5 d. Bar, 50 µm.