Figure 4.

OT-1 cell concentration determines the efficiency of killing of SIINFEKL peptide–pulsed B16 cells in packed cell pellet–type assays. (A) 10⁴ SIINFEKL-pulsed (♦) or nonpulsed (■) B16 cells were mixed with 10–5 × 10⁵ activated OT-1 cells in 200 µl OT-1 medium in round-bottom wells of a 96-well plate. Plates were centrifuged at 50 g for 5 min to pellet the cells and incubated at 37°C for 4 h. Cells in each well were dissociated with trypsin-EDTA, and their viability was measured by clonogenic assay. Data shown represent mean ± SEM of n = 3 experiments performed in duplicate. (B) Efficiency of OT-1 cell killing of SIINFEKL-B16 cells in collagen-fibrin gels (▴) versus packed pellet–type assays (♦). Data for collagen-fibrin gels were obtained from Table S1. Data for packed pellet–type assays were obtained from A.