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. 2009 Oct 20;87(2):257–263. doi: 10.1189/jlb.0409221

Figure 2.

Figure 2.

The activation of ERK is required for CD8+ effector maturation and the expression of IL-12Rβ2 in CD8+ T cells. (A and B) Naive OT-I T cells preincubated 1 h with U0126 (10 μM), SB203580 (10 μM), or SP600125 (10 μM) were cultured with BOK cells for an additional 20 h in the presence or absence of IL-12 (2 ng/ml). OT-I T cells were transferred, rested, and then harvested at 72 h. The percent of IFN-γ+CD8+ T cells was determined by ICS and flow cytometry analysis at 72 h (A). The cytolylic ability was evaluated in a standard 4-h chromium release assay (B). (C) Intracellular phospho-ERK (pERK) staining of CD8+ OT-I T cells stimulated with 16.7% or 100% ratio of APCs for indicated times. (D and E) Purified, naïve OT-I CD8+ T cells that had been incubated for 1 h with U0126 (10 μM) or DMSO were cultured for the indicated condition. (D) RT-PCR analysis of IL-12β2 mRNA at 1 h or 20 h after stimulation. The level of β-actin mRNA was used as an internal control. (E) Surface expression of IL-12Rβ2 on CD8+ gated OT-I T cells at 48 h. *, P < 0.05; **, P < 0.01. The results are representative of three independent experiments.