Figure 3.

Ets-1 activation is required for CD8+ T cells effector function and survival. (A) Purified, naïve OT-I CD8+ T cells were stimulated with PMA (20 μM) or low or high frequency of APCs, with or without U0126 (10 μM). Cells were lysed after 15 min or 60 min. Induction of Ets-1 phosphorylation (p-Ets-1) was detected by Western blot analysis. (B–D) CD8+ T cells derived from wild-type (WT) and Ets-1-deficient [knockout (KO)] mice were stimulated with anti-CD3 mAb (1 μg/ml) and anti-CD28 mAb (0.5 μg/ml). (B) RT-PCR analysis of indicated genes at 48 h. (C) ICS and flow cytometry analysis for IFN-γ at 72 h. (D) Percent cell recovery at 72 h. *, P < 0.05; **, P < 0.01. The results are representative of three independent experiments.