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. 2009 Nov 4;87(2):301–308. doi: 10.1189/jlb.0409238

Figure 4.

Figure 4.

The effects of IL-23 and IL-10 exposure on cIELs from IL-10−/− mice were retained following recall stimulation. For recall stimulation, cIELs from IL-10−/− mice stimulated in vitro for 24 h with 1 μg/ml plate-bound anti-CD3 antibody in the presence of medium, medium plus rIL-23, or medium plus rIL-10. Recall stimulation in the presence of rIL-23 increased the percent of IL-17+ cells in the ICOS+ population, whereas IL-10 exposure decreased the percent of IL-17+ cells. Similarly, the MFI of ICOS expression was increased by exposure to rIL-23 and decreased by IL-10 exposure relative to cells cultured in medium alone. The high percentage of IL-17+ IFN-γ+-positive cells in the ICOS– population may be a result of autofluorescence of dead cells. Data are representative of two independent experiments.