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. 2009 Nov 4;87(2):301–308. doi: 10.1189/jlb.0409238

Figure 6.

Figure 6.

IL-17 expression and pathology are regulated by ICOS signaling. (A) Bone marrow DCs express the B7RP-1 ICOS ligand. (B) cIELs from normal BALB/c mice cultured with IL-17-inducing cocktail in the presence of DCs (media plus DCs) produced more IL-17A as determined by ELISA than cIELs cultured with IL-17-producing cocktail alone (media). No IL-17A was produced by DCs by themselves when cultured with IL-17-inducing cocktail (DCs plus media); data are mean values of three replicate samples. (C) cIELs cultured in media plus DCs resulted in a statistically significant increase in IL-17A production. Culture of cIELs from ICOS−/− mice with B7RP-1+ DCs failed to result in elevated levels of IL-17A production. Data for A–C are representative of two to three independent experiments. (D) IL-10−/− mice treated for 10 days with 10 μg anti-ICOS antibody (n=4) have statistically significant, lower pathology scores compared with mice treated with 10 μg isotype control Ig (n=4).