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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 10;391(1):235–241. doi: 10.1016/j.bbrc.2009.11.039

FIGURE 3. LPS induced attachment and β1 integrin up-regulation.

FIGURE 3

(A) Attachment assay of H69 treated with LPS (1 μg/ml). Cells were transfected with either EV or IκBα-M expression plasmid and ß-galactosidase reporter plasmid, and then plated in fibronectin-coated wells. The β-galactosidase activity reflects the amount of attachment of transfected cells as described in the Material and Methods section. (B) Western blot analysis of H69 with LPS (1μg/ml). The membrane was probed with the indicated antiserum at right. (C) RTq-PCR analysis of H69 cells cultured in uncoated wells. Cells were treated with LPS (1μg/ml) for 24 hours in uncoated wells. The fold induction represents the amount of b1 integrin induction over the H69 without LPS.