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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Biochem Pharmacol. 2009 Oct 27;79(5):784–791. doi: 10.1016/j.bcp.2009.10.007

Fig. 6. Nondenaturing gradient gel stained for esterase activity (A) and counterstained with Coomassie Blue (B).

Fig. 6

Lanes 1 and 10, control human plasma (5 μl), 0 mM aspirin. Lanes 2-9 contain 200 μg of human albumin treated for 24 h with aspirin. Lane 2, 0 mM aspirin; Lane 3, 1 mM aspirin; Lane 4, 10 mM aspirin; Lane 5, 20 mM aspirin; Lane 6, 30 mM aspirin; Lane 7, 40 mM aspirin; Lane 8, 50 mM aspirin; Lane 9, 0 mM aspirin. Albumin esterase activity in panel A diminishes with increasing aspirin concentrations. Acetylated albumin in lane 8 migrates more rapidly than untreated albumin in lane 9. The arrow shows the direction of migration of proteins on the gel.