Figure 1.

(A) Northern blot hybridization of VEGF, VEGFR-1, and -2 transcripts in adult mice tissues. Total RNA (20 μg) from normoxic and hypoxic (6% O₂ for 6 h) organs was hybridized to the indicated ³²P-labeled cDNA probes and exposed to a BioImager screen (BAS-2500, Fuji). (B) PCR analysis of VEGF transcripts (VEGF₁₈₈, 716 bp; VEGF₁₆₄, 644 bp; VEGF₁₂₀, 512 bp). cDNA was synthesized from 2 μg of total RNA from normoxic and hypoxic organs. −, no RT; +, positive control (plasmid VEGF₁₆₄); m, molecular weight marker (506 bp).