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Localization of VEGF (a, b, e, f) and VEGFR-1 (c, g) mRNA and PECAM-1 protein (d, h) on serial sections from normoxic (a–d) and hypoxic (CO-treated) (e–h) brain. A cortical microvessel is shown on bright-field (a, e) and dark-field images (b, c, f, g) from in situ hybridization experiments and immunohistochemistry images (d, h). Note that endothelial cells do not express VEGF.
