Figure 1.

Western blot of P-gp protein expression in human placental brush border membranes in representative wild type and variants representing 1236T-3435T-2677T/A genotype (10 μg/lane). The immunoblot was probed with the P-gp monoclonal antibody C219. Immunoreactive protein bands detected by immunoblotting were analyzed by densitometry. P-gp expression was determined as a proportion of the total amount of β-actin present per lane.