Figure 1. Phosphorylation at homologous residues on α-CaMKII and β-CaMKII under different incubation conditions.
PSD fractions, incubated under phosphorylating conditions as summerized above, were digested with trypsin, the four samples within each group were differentially labeled with iTRAQ reagents. Phosphopeptides were separated by TiO2 chromatography and analyzed by LC/MS/MS. Relative abundance of phosphopeptides were estimated by normalizing corresponding total counts with respect to sample 2. Two experiments were carried out for each protocol. Values from both experiments are shown when available.
