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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 5;391(1):78–84. doi: 10.1016/j.bbrc.2009.10.167

Figure 2. SynGAP is most efficiently phosphorylated by the autonomous form of CaMKII in the absence of Ca2+.

Figure 2

PSD samples ware phosphorylated under four different conditions as follows: Sample 1: 5min on ice in Ca2+; Sample 2: 5min on ice in Ca2+,10 min at 37°C in Ca2+; Sample 3: 5 min on in ice Ca2+, 10 min at 37°C in EGTA; Sample 4: 5 min on ice in EGTA, 10 min at 37°C in EGTA. Relative phosphorylation levels for all three residues indicate highest levels of phosphorylation in Sample 3, corresponding to autonomous activity in the absence of Ca2+.