TABLE 2.
Effect of endogenous phosphatase activity on the phosphorylation levels at identified residues at the PSD.
| Protein Accession number |
Phosphorylated Peptide | Residue | % Phosphorylation in the presence vs. absence of phosphatase inhibitor |
|---|---|---|---|
|
Densin-180 UniProtKB: P70587 |
R.IVGVPLELEQ[ST]*HR.H | S-831 or T-832 |
51% 64% |
|
SAPAP1 UniProtKB: P97836 |
K.ATQP[SLT]*ELTTLK.I | S-389 or T-391 |
60% |
|
SAPAP1 UniProtKB: P97836 |
K.FQS*VGVQVEEEK.C | S-696 | 45% |
|
SAPAP2 UniProtKB: P97837 |
R.EKS*LDLPDR.Q | S-1012 | 76% 80% |
|
SAPAP3 UniProtKB: P97838 |
K.ERS*LDSVDR.Q | S-930 | 53% 58% |
|
Shank1 UniProtKB: Q9WV48 |
K.RLPPPAIS*LR.S | S-783 | 31% 54% |
|
Shank3 UniProtKB: Q9JLU4 |
R.SK[SMT]*AELEELASIR.R | S-769 or T-771 |
85% 59% |
|
Shank3 UniProtKB: Q9JLU4 |
R.S*LGEEPVGGLGSLLDPAK.K | S-1586 | 89% 86% |
|
Spinophilin UniProtKB: O35274 |
R.A[SS]*LNENVDHSALLK.L | S-99 or S-100 |
41% |
|
SynGAP UniProtKB: Q9QUH6 |
R.DLN[SS]*IDLQSFMAR.G | S-765 or S-766 |
64% 81% |
|
SynGAP UniProtKB: Q9QUH6 |
R.GKS*QQLTVSAAQKPR.P | S-1073 | 83% 72% |
|
SynGAP UniProtKB: Q9QUH6 |
K.EG[SIGGS]*GGSGGGGGGGLKPSITK.Q | S1114 or S1118 |
116% 88% |
PSD samples were incubated at 37°C for 10 min in media containing Ca2+ with or without phosphatase inhibitors (MicrocystinLR and NaF).