Fig. 2.
Spectra of purified P450 4F11. (A) Absorbance spectra of P450 4F11 (ferric and ferrous spectra). The P450 concentration was 2.0 μM, and the ferrous spectrum was recorded following reduction with Na2S2O4. (B) Second derivative of absorbance spectrum (ferric state). (C) Fe2+•CO vs. Fe2+ difference spectrum. The P450 concentration was 2.2 μM (⌊max 451 nm).
