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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Arch Biochem Biophys. 2009 Nov 20;494(1):86–93. doi: 10.1016/j.abb.2009.11.017

Table 2.

Purification of human P450 4F11 from E. coli membrane fractions.

Purification step P450 (nmol) Yield (%)
Membranes 227 100
Solubilized fractiona 214 94
Ni2+-NTA column fractionb 106 47
Hydroxylapatite column fractionc 73 32
a

Solubilization using a mixture of sodium cholate plus Tergitol NP-10, followed by centrifugation to remove insoluble material.

b

P450 eluted with 200 mM imidazole.

c

Eluted with 0.7 M potassium phosphate buffer (pH 7.7) containing 0.2% sodium cholate (w/v).