Table 3.

Spectral titration of human P450 4F11 with fatty acids.

Fatty acid	ΔAmax	Ksa (μM)
C16:0	0.047 ± 0.006 b	40 ± 11
C18:1	0.037 ± 0.003 c	71 ± 14
C20:4	-d	-
C22:6	-	-
3-OH C16:0	0.062 ± 0.006 b	69 ± 14
	0.065 ± 0.006 c	98 ± 16

^aSee Supplemental Data, Fig. S1. Estimated from the hyperbolic fitting plot of ΔA_max versus the fatty acid concentration using a hyperbolic equation in GraphPad Prism software (GraphPad Software, San Diego, CA).

^bDifference in absorbance between the absorbance maximum at 388 nm and minimum at 418 nm, ΔA₃₈₈ − A₄₁₈.

^cDifference in absorbance between the absorbance maximum at 438 nm and minimum at 418 nm, ΔA₄₃₈ − A₄₁₈.

^d–, no detectable binding (ΔA₃₈₈ < 0.005 at 50 μM).