Table 5.
Steady-state kinetics analysis of oxidations of fatty acids by human P450 4F11.
| Fatty acid | kcat (min−1) | Km (μM) | kcat/Km (min−1 μM−1) |
|---|---|---|---|
| C16:0 | 1.8 ± 0.1 | 47 ± 4 | 0.038 ± 0.004 |
| C18:1 | 1.0 ± 0.1 | 85 ± 10 | 0.012 ± 0.002 |
| C20:4 | 0.54 ± 0.06 | 80 ± 21 | 0.007 ± 0.002 |
| C22:6 | 0.35 ± 0.04 | 48 ± 14 | 0.007 ± 0.002 |
| 3-OH C16:0 | 27.5 ± 4.5 | 74 ± 26 | 0.37 ± 0.14 |