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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 13;391(1):299–304. doi: 10.1016/j.bbrc.2009.11.053

Figure 2. Delayed muscle cell differentiation by NS depletion.

Figure 2

(A) Western blotting of Day 0 myoblast extract after transfection of siRNAs.

(B) Differentiation pattern of muscle cells after siRNA transfection. Cells were double immunostained with anti-NS antibody and anti-MHC antibody. Bar, 40 μm. (C) Mean + SEM of the ratio between the number of DAPI-positive nuclei in MHC-positive cells and the total number of DAPI-positive nuclei shown in (B) obtained from three independent experiments. Two hundred nuclei were counted in each experiment. Asterisks indicate pairs where differences were statistically significant (t-test, P < 0.05).

(D) Autoradiograph of an SDS-PAGE gel loaded with Day 0 myoblast extracts after siRNA transfection. These myoblasts were metabolically labeled with 35S methionine for 4 hrs while cultured in the growth medium.

(E) Relative intensity of the total radioactivity in each lane shown in (D). Each lane in the dried gel was excised and the radioactivity was measured with a scintillation counter. Radioactivity of the control siRNA lane was defined as 100%.