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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 13;391(1):299–304. doi: 10.1016/j.bbrc.2009.11.053

Figure 3. Facilitation of muscle cell differentiation by overexpressed NS.

Figure 3

(A) Myoblasts infected with the empty or the NS-expressing retrovirus were immunostained with anti-6xHis tag antibody to detect NS. Bar, 10 μm.

(B) Comparison of cell proliferation rates between myoblasts infected with the empty retrovirus and the NS-expressing retrovirus. The cells were cultured in the growth medium.

(C) Immunostaining of muscle cells with anti-MHC antibody after infection with the empty or the NS-expressing retrovirus. The cells were cultured in the differentiation medium. Bar, 20 μm.

(D) Mean + SEM of the ratio between the number of DAPI-positive nuclei in MHC-positive cells and the total number of DAPI-positive nuclei shown in (C) obtained from three independent experiments. One hundred nuclei were counted in each experiment.

(E) Western blotting of extracts prepared from muscle cells infected with the empty or the NS-expressing retrovirus. β actin was monitored as a loading control.

(F) Relative expression levels of three mRNAs measured by qRT-PCR after overexpression of NS. The value with the empty virus on Day 0 was defined as 1 for each mRNA.